Biophysicists from the Moscow Institute of Physics and Technology have
joined forces with colleagues from France and Germany to create a new
fluorescent protein. Besides glowing when irradiated with ultraviolet and blue
light, it is exceedingly small and stable under high temperatures. The authors
of the paper, published in the journal Photochemical &
Photobiological Sciences, believe the protein holds prospects for
fluorescence microscopy. This technique is used in research on cancer,
infectious diseases, and organ development, among other things.
Fluorescence microscopy is a method for studying living tissue that relies
on induced luminescence. After being exposed to laser radiation at a particular
wavelength, some proteins emit light at a different wavelength. This induced
“glow” can be analyzed using a special microscope. Researchers append such
fluorescent proteins to other proteins via genetic engineering to make the
latter ones visible to the microscope and observe their behavior in cells.
Fluorescence microscopy proved so scientifically valuable that one Nobel Prize
was awarded for its discovery, followed by another one for radically improving
the method’s accuracy.
Up until now, the fluorescent proteins used for such observations had
several flaws. They were vulnerable to heat, fairly bulky, and only glowed in
the presence of oxygen.
“For one thing, our protein is more thermostable than its analogues: It
only denaturates at 68 degrees Celsius,” said the paper’s lead author Vera
Nazarenko from the MIPT Laboratory of Structural Analysis and Engineering of Membrane
Systems. “It is also miniature, while most of the currently used fluorescent
proteins are rather bulky. On top of that, it can emit light in the absence of
oxygen.”
The team originally identified the protein with these remarkable properties
in the cells of a the thermophilic bacterium — that is, one which lives in
high-temperature environments, such as hot springs. The researchers then
genetically engineered a DNA sequence that reproduced the protein’s fluorescent
segment but not the other parts, which would make the molecule larger.
By introducing the gene that encodes the protein into the cells of another
bacterium, Escherichia coli, the team turned it into a factory mass-producing
the fluorescent protein with unique properties.
Researchers studying the processes that occur in living cells have been
waiting for a protein combining these crucial features for a long time. By
introducing it into cells, they can now obtain essential data on cell life and
death. To name a few applications, fluorescence microscopy is seen as one of
the best tools for investigating the mechanism behind malignant tumor genesis
and development. It is also useful for research on cell signaling and organ
development.
The proteins previously used in fluorescence microscopy were bulky and
thermally unstable, putting limitations on the method. Thanks to the MIPT team,
that obstacle has been eliminated.
Journal article: https://pubs.rsc.org/en/content/articlelanding/2019/pp/c9pp00067d/unauth#!divAbstract
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